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Publications

Note: In publication lists the names of my undergrad & grad students are underlined, with undergrads in italics font style.

Original Research Publications | Review Articles & Book Chapters | Books Edited | Published Conference Proceedings

Original Research Publications        

  1. Menard BS, Raytek LM, Benidickson KH, Snedden WA, Plaxton WC (2024) Heterologous expression and purification of glutamate decarboxylase-1 from the model plant Arabidopsis thaliana: characterization of the enzyme’s in vitro truncation by thiol endopeptidase activity. Protein Expression & Purification (in press; https://doi.org/10.1016/j.pep.2024.106612).
  2. Symonds K, Smith MA, Esme O, Plaxton WC, Snedden WA (2024) Characterization of Arabidopsis aldolases AtFBA4 and AtFBA5; inhibition by morin and interaction with calmodulin. FEBS Letters 598: 1864-1876; https://doi.org/10.1002/1873-3468.14979
  3. Smith MA, Benidickson KH, Plaxton WC (2024) In vivo phosphorylation of the cytosolic glucose-6-phosphate dehydrogenase isozyme G6PD6 in phosphate-resupplied Arabidopsis thaliana suspension cells and seedlings. Plants  13; 31; https://doi.org/10.3390/plants13010031                                                                                                                     Graphical Abstract

     

  4. Benidickson KH  Raytek LM, Hoover GJ, Flaherty EJ, Shelp BJ, Snedden WA, Plaxton WC (2023) Glutamate decarboxylase-1 is essential for efficient acclimation of Arabidopsis thaliana to nutritional phosphorus deprivation. New Phytologist  240: 2372-2385; https://doi.org/10.1111/nph.19300
  5. Hau B, Symonds K, Teresinski H, Janssen A, Duff L, Smith MA, Benidickson KH, Plaxton WC, Snedden WA (2023) Arabidopsis calmodulin-like proteins CML13 and CML14 interact with calmodulin-binding transcriptional activators and function in salinity stress response. Plant & Cell Physiology 65::282-300; https://doi.org/10.1093/pcp/pcad152
  6. Symonds K, Teresinski H, Hau B, Chiasson D, Benidickson KH, Plaxton WC, Snedden WA (2023) Arabidopsis CML13 and CML14 have essential and overlapping roles In plant development. Plant & Cell Physiology 65: 228-242;  https://doi.org/10.1093/pcp/pcad142
  7. Kilburn R, Fedosejevs ET, Mehta D, Soleimani F, Ghahremani M, Monaghan J, Thelen JJ, Uhrig RG, Snedden WA, Plaxton WC (2023) Substrate profiling of the Arabidopsis Ca2+-dependent protein kinase AtCPK4 and its Ricinus communis ortholog RcCDPK1. Plant Science 331: 11675; https://doi.org/10.1016/j.plantsci.2023.111675
  8. Kilburn R, Gerdis SA, She Y-M, Snedden WA, Plaxton WC (2022) Autophosphorylation inhibits RcCDPK1, a dual-specificity kinase that phosphorylates bacterial-type phosphoenolpyruvate carboxylase in castor oil seeds. Plant Cell & Physiology 63: 683–698; https://doi.org/10.1093/pcp/pcac030
  9. O’Gallagher  B, Ghahremani M, Stigter K, Walker E, Pyc M, Liu A-Y, MacIntosh GC,Mullen RT,Plaxton WC (2021) Biochemical and molecular characterization of AtPAP17: a dual-localized, low molecular weight Arabidopsis purple acid phosphatase upregulated during phosphate deprivation, senescence, and oxidative stress. Journal of Experimental Botany. 73: 382–399; https://doi.org/10.1093/jxb/erab409
  10. Mehta D, Ghahremani M, Pérez Fernández M, Tan M, Schläpfer P, Plaxton WC, Uhrig RG (2021) Phosphate and phosphite differentially impact the proteome and phosphoproteome of Arabidopsis suspension cell cultures. The Plant Journal 105: 924-941
  11. Ghahremani M, Park J, Anderson EM, Marty-Howard NJ, Mullen RT, Plaxton WC (2019) Lectin AtGAL1 interacts with high-mannose glycoform of the purple acid phosphatase AtPAP26 secreted by phosphate-starved Arabidopsis. Plant Cell Environment 42:1158–1166
  12. Ghahremani M, Tran H, Biglou SG, O'Gallagher B, She Y-M, Plaxton WC (2019)  A glycoform of the purple acid phosphatase AtPAP26 co-purifies with a mannose-binding lectin (AtGAL1) secreted by phosphate-starved Arabidopsis. Plant Cell Environment 42:1139–1157. **winner of 2020 Ragai Ibrahim award of Can Soc Plant Biol, for 'best original research publication by a CSPB student member that appeared in the preceding calendar year'   
  13. Willick IR, Plaxton WC, Lolle SJ, Macfie SM (2019) Transcriptional and post-translational upregulation of phosphoenolpyruvate carboxylase in Arabidopsis thaliana under cadmium stress. Environmental & Experimental Botany 60: 715–724
  14. Connell MB, Lee MJY, Li J, Plaxton WC, Jia Z (2018) Structural and biochemical characterization of citrate binding to AtPPC3, a plant-type phosphoenolpyruvate carboxylase from Arabidopsis thaliana. Journal of Structural Biology 204: 507-512 
  15. Fedosejevs ET, Feil R, Lunn J, Plaxton WC (2018) The signal metabolite trehalose-6-phosphate inhibits the sucrolytic activity of sucrose synthase from developing castor beans. FEBS Letters 592: 2525–2532 (selected as EDITOR'S CHOICE)
  16. Ting MK, She Y-M, Plaxton WC (2017) Transcript profiling indicates a widespread role for bacterial-type phosphoenolpyruvate carboxylase in malate-accumulating sink tissues. Journal of Experimental Botany  68: 5857–5869
  17. Fedosejevs ET, Liu LN, Abergel M, She Y-M., Plaxton WC (2017) Coimmunoprecipitation of reversibly glycosylated polypeptide with sucrose synthase from developing castor oilseeds. FEBS Letters  591: 3872–3880
  18. Ying S, Hill A, Pyc M, Anderson E, Snedden WA, Mullen RT, She Y-M, Plaxton WC (2017) Regulatory phosphorylation of bacterial-type PEP carboxylase by the Ca2+-dependent protein kinase RcCDPK1 in developing castor oilseeds. Plant Physiology 174: 1012-1027.
  19. Unterlander N, Champagne P, Plaxton WC (2017) Lyophilization pretreatment facilitates extraction of soluble proteins and active enzymes from the oil-accumulating microalga Chlorella vulgaris. Algal Research 25: 439-444
  20. Fedosejevs ET, Gerdis SA, Ying S, Pyc M, Anderson EM, Snedden WA, Mullen RT, She Y-M, Plaxton WC (2016) The calcium-dependent protein kinase RcCDPK2 phosphorylates sucrose synthase at Ser11 in developing castor oil seeds. Biochemical Journal 473: 3667–3682
  21. Ruiz-Ballesta I, Baena G, Gandullo J, Wang L, She Y-M, Plaxton WC, Echevarría C (2016) New insights into the post-translational modification of multiple phosphoenolpyruvate carboxylase isoenzymes by phosphorylation and monoubiquitination during sorghum seed development and germination. Journal of Experimental Botany 67: 3523–3536
  22. Shane, MW, Feil R, Lunn JE, Plaxton WC (2016) Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply. Annals of Botany 118: 637–643
  23. Figueroa CM, Feil R, Ishihara H, Watanabe M, Kölling K, Krause U, Höhne M, Encke B, Plaxton WC, Zeeman SC, Li Z, Schulze WX, Hoefgen R, Stitt M, Lunn JE (2016) Trehalose 6-phosphate coordinates organic and amino acid metabolism with carbon availability.            The Plant Journal 85: 410-423
  24. Fedosejevs ET, Ying S, Park J, Anderson EM, Mullen RT, She Y-M, Plaxton WC (2014) Biochemical and molecular characterization of RcSUS1, a cytosolic sucrose synthase phosphorylated in vivo at serine-11 in developing castor oilseeds. Journal of Biological Chemistry 289: 33412-33424
  25. Del Vecchio HA, Ying S, Park J, Knowles VL,  Kanno S,  Tanoi K, She Y-M, Plaxton WC (2014) The cell-wall targeted purple acid phosphatase AtPAP25 is critical for acclimation of Arabidopsis thaliana to nutritional phosphorus-deprivation. The Plant Journal 80: 569-581 (selected as a 'Featured  Publication') PJ cov
  26. Hill AT, Ying S, Plaxton WC (2014) Phosphorylation of bacterial-type phosphoenolpyruvate carboxylase by a Ca2+-dependent protein kinase suggests a link between Ca2+-signaling and anaplerotic pathway control in developing castor oil seeds. Biochemical Journal 458: 109-118
  27. Shane MW, Stigter K, Fedosejevs ET, Plaxton WC (2014) Senescence-inducible cell-wall and intracellular purple acid phosphatases: implications for phosphorus remobilization in Hakea prostrata (Proteaceae) and Arabidopsis thaliana (Brassicaceae). Journal of Experimental Botany 65: 6097-6106  jxb cov
  28. Dahal K, Knowles VL, Plaxton WC, Hüner NPA (2014) Enhancement of photosynthetic performance, water use efficiency and grain yield during long-term growth under elevated CO2 in wheat and rye is growth temperature and cultivar dependent. Environmental and Experimental Botany 106:  207-220
  29. Ruiz-Ballesta I, Feria A-B, Hong N, She Y-M, Plaxton WC, Echevarria C (2014) In vivo monoubiquitination of anaplerotic phosphoenolpyruvate carboxylase occurs at lysine-624 in germinating sorghum seeds. Journal of Experimental Botany 65: 443-451
  30. Dahal K, Sarathi MW, Kane K, Rauf SA, Leonardos ED, Gadapati W, Savitch L, Singh J, Marillia E-F, Taylor DC, Micallef MC, Knowles VL, Plaxton WC, Barron J, Sarhan F, Huner N, Grodzinski B, Micallef BJ (2014) Enhancing biomass production and yield by maintaining enhanced capacity for CO2 uptake in response to elevated CO2. Canadian Journal of Plant Science 94: 1075-1083
  31. Shane MW, Fedosejevs ET, Plaxton WC (2013) Reciprocal control of anaplerotic phosphoenolpyruvate carboxylase by in vivo monoubiquitination and phosphorylation in developing proteoid roots of phosphate deficient Hakea prostrata. Plant Physiology 161:1634–1644   shane cov   
  32. Robinson WD, Park J, Tran HT, Del Vecchio HA, Ying S, Patel K, McKnight TD, Plaxton WC (2012) The secreted purple acid phosphatase isozymes AtPAP12 and AtPAP26 play a pivotal role in extracellular phosphate scavenging by Arabidopsis thaliana. Journal of Experimental Botany 63: 6531–6542
  33. Robinson WD, Carson I, Ying S, Ellis K, Plaxton WC (2012) Eliminating the purple acid phosphatase AtPAP26 in Arabidopsis thaliana delays leaf senescence and impairs phosphorus remobilization. New Phytologist 196: 1024–1029
  34. Park J, Khuu N, Howard AS, Mullen RT, Plaxton WC (2012) Bacterial- and plant-type phosphoenolpyruvate carboxylase isozymes from developing castor oil seeds interact in vivo and associate with the surface of mitochondria. The Plant Journal 71: 251-262
  35. Dalziel KJ, O'Leary B, Brikis C, Rao SK, She Y-M, Cyr T, Plaxton WC (2012) The bacterial-type phosphoenolpyruvate carboxylase   isozyme from developing castor oil seeds is subject to in vivo regulatory phosphorylation at serine-451. FEBS Letters 586: 1049-1054
  36. O'Leary B, Fedosejevs ET, Hill AT, Bettridge J, Park J, Rao SK, Leach CA, Plaxton WC (2011) Tissue-specific expression and post-translational modifications of plant- and bacterial-type phosphoenolpyruvate carboxylase isozymes of the castor oil plant, Ricinus communis L. Journal of Experimental Botany 62: 5485-5495
  37. O'Leary B, Rao S, Plaxton WC (2011) Phosphorylation of a bacterial-type phosphoenolpyruvate carboxylase at serine-425 provides a further tier of enzyme control in developing castor oil seeds. Biochemical Journal 433: 65-74
  38. Tran HT, Qian W, Hurley BA, She Y-M, Wang D, Plaxton WC (2010) Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatases isozymes secreted by phosphate-starved Arabidopsis thaliana. Plant, Cell & Environment 33: 1789-1803
  39. Hurley BA, Tran HT, Murty N, Park J, Snedden WA, Mullen RT, Plaxton WC (2010) The dual-targeted purple acid phosphatase isozyme AtPAP26 is essential for efficient acclimation of Arabidopsis thaliana to nutritional phosphate deprivation. Plant Physiology 153:1112–1122
  40. O'Leary B, Rao S, Kim J, Plaxton WC (2009) Bacterial-type phosphoenolpyruvate carboxylase (PEPC) functions as a catalytic and regulatory subunit of the novel class-2 PEPC complex of vascular plants. Journal of Biological Chemistry 284: 24797-243805
  41. Gregory AL, Hurley BA, Tran HT, Valentine AJ, She Y-M, Knowles VL, Plaxton WC (2009) In vivo regulatory phosphorylation of the phosphoenolpyruvate carboxylase AtPPC1 in phosphate-starved Arabidopsis thaliana. Biochemical Journal 420: 57-65
  42. Uhrig RG, She Y-M, Leach CA, Plaxton WC (2008) Regulatory monoubiquitination of phosphoenolpyruvate carboxylase in germinating castor oil seeds. Journal of Biological Chemistry 283: 29650-29657 (selected as a JBC 'Paper of the Week').  jbc cov
  43. Tran HT, Plaxton WC (2008) Proteomic analysis of alterations in the secretome of Arabidopsis thaliana suspension cells subjected to nutritional phosphate deficiency. Proteomics 8: 4317-4326
  44. Uhrig RG, O’Leary B, Spang HE, MacDonald JA, She Y-M, Plaxton WC (2008) Co-immunopurification of phosphorylated bacterial- and plant-type phosphoenolpyruvate carboxylases with the plastidial pyruvate dehydrogenase complex from developing castor oil seeds. Plant Physiology 146: 1346-1357
  45. Gennidakis S, Rao S, Greenham K, Uhrig RG, O’Leary B, Snedden WA, Lu C, Plaxton WC (2007) Bacterial- and plant-type phosphoenolpyruvate carboxylase polypeptides interact in the hetero-oligomeric Class-2 PEPC complex of developing castor oil seeds. The Plant Journal 52: 839–849
  46. Murmu J, Plaxton WC (2007) Phosphoenolpyruvate carboxylase protein kinase from developing castor oil seeds: Partial purification, characterization, and reversible control by photosynthate supply. Planta 226: 1299-1310
  47. Martin M, Plaxton WC, Podestà FE (2007) Activity and concentration of non-proteolyzed phosphoenolpyruvate carboxykinase in the endosperm of germinating castor oil seeds: effects of anoxia on its activity. Physiologia Plantarum 130: 484-494
  48. Bozzo GG, Dunn EI, Plaxton WC (2006) Differential expression of phosphate-starvation inducible purple acid phosphatase isozymes in tomato (Lycopersicon esculentum). Plant, Cell & Environment 29: 303-313
  49. Veljanovski V, Vanderbeld B, Knowles VL, Snedden WA, Plaxton WC (2006) Biochemical and molecular characterization of AtPAP26, a vacuolar purple acid phosphatase upregulated in phosphate-deprived Arabidopsis suspension cells and seedlings. Plant Physiology 142: 1282-1293
  50. Crowley V, Gennidakis S, Plaxton WC (2005) In vitro proteolysis of phosphoenolpyruvate carboxylase from developing castor oil seeds by an endogenous thiol endopeptidase. Plant Cell Physiology 46: 1855-1862
  51. Tripodi K, Gennidakis S, Turner WL, Plaxton WC (2005) In vivo regulatory phosphorylation of novel phosphoenolpyruvate carboxylase isoforms in endosperm of developing castor oil seeds. Plant Physiology 139: 969-978
  52. Turner WL, Knowles VL, Plaxton WC (2005) Cytosolic pyruvate kinase subunit composition, activity, and amount in developing castor and soybean seeds, and biochemical characterization of the purified castor seed enzyme. Planta 222: 1051-1062
  53. Weese B, Plaxton WC (2005) Purification and characterization of a homodimeric enolase from the cyanobacterium Synechococcus sp. PCC 6301. Journal of Phycology 41: 515-522
  54. Lal AK, Plaxton WC, Kayastha AM (2005) Purification and characterization of an allosteric fructose-1,6-bisphosphate aldolase from mung beans. Phytochemistry 66: 968-974
  55. Bozzo GG, Singh V, Plaxton WC (2004) Phosphate or phosphite addition promotes the proteolytic turnover of phosphate-starvation inducible tomato purple acid phosphatase isozymes. FEBS Letters 573: 51-54
  56. Bozzo GG, Raghothama KG, Plaxton WC (2004) Structural and kinetic properties of a novel purple acid phosphatase from phosphate-starved tomato (Lycopersicon esculentum) cell cultures. Biochemical Journal 377: 419-428
  57. Blonde JD, Plaxton WC (2003) Structural and kinetic properties of high and low molecular mass phosphoenolpyruvate carboxylase isoforms from the endosperm of developing castor oil seeds. Journal of Biological Chemistry 278: 11867-11873
  58. Singh V, Knowles VL, Wood S, Plaxton WC (2003) Phosphite-accelerated programmed cell death in phosphate-starved oilseed rape (Brassica napus) suspension cell cultures. Planta 218: 233-239
  59. Podestà FE, Plaxton WC (2003) Fluorescence study of ligand binding to potato tuber pyrophosphate-dependent phosphofructokinase: evidence for competitive binding between fructose-1,6-bisphosphate and fructose-2,6-bisphosphate. Archives of Biochemistry & Biophysics 414:101-107
  60. Knowles VL, Plaxton WC (2003) From genome to enzyme: Quantitative analysis of glycolytic and oxidative pentose pathway enzymes from the cyanobacterium Synechocystis PCC 6803. Plant Cell Physiology 44: 758-763
  61. Turner WL, Plaxton WC (2003) Purification and characterization of pyrophosphate- and ATP-dependent phosphofructokinases from banana fruit. Planta 217: 113-121
  62. Bozzo GG, Raghothama KG, Plaxton WC (2002) Purification and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato cell cultures. European Journal of Biochemistry 269: 6278-6287
  63. Rivoal J, Turpin DH, Plaxton WC (2002) In vitro phosphorylation of phosphoenolpyruvate carboxylase from the green alga Selenastrum minutum. Plant Cell Physiology 43: 785-792
  64. Plaxton WC, Smith CR, Knowles VL, (2002) Molecular and regulatory properties of leucoplast pyruvate kinase from Brassica napus (rapeseed) suspension cells. Archives of Biochemistry and Biophysics 400: 54-62
  65. Rivoal J, Smith CR, Moraes TF, Turpin DH, Plaxton WC (2002) Enzyme activity staining after native polyacrylamide gel electrophoresis using fluorescence detection. Analytical Biochemistry 300: 94-99
  66. McDonald AE, Niere JO, Plaxton WC (2001) Phosphite disrupts the acclimation of Saccharomyces cerevisiae to phosphate starvation. Canadian Journal of Microbiology 47: 969-978
  67. Turner WL, Plaxton WC (2001) Purification and characterization of banana fruit acid phosphatase. Planta 214: 243-249
  68. Knowles VL, Smith CR, Smith CS, Plaxton WC (2001) Structural and regulatory properties of pyruvate kinase from the cyanobacterium Synechococcus PCC 6301. Journal of Biological Chemistry 276: 20966-20972
  69. Rivoal J, Trzos S, Gage DA, Plaxton WC, & Turpin DH (2001) Two unrelated phosphoenolpyruvate carboxylase polypeptides physically interact in the high molecular mass isoform of this enzyme in the unicellular green alga Selenastrum minutum. Journal of Biological Chemistry 276: 12588 12597
  70. Palma DA, Blumwald E, Plaxton WC (2000) Upregulation of vacuolar H+-translocating pyrophosphatase by phosphate starvation of Brassica napus (rapeseed) suspension cell cultures. FEBS Letters 486, 155-158
  71. Turner WL, Plaxton WC (2000) Purification and characterization of cytosolic pyruvate kinase from banana fruit. Biochemical Journal 352, 875-882
  72. Moraes T, Plaxton WC (2000) Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (rapeseed) suspension cell cultures. Implications for phosphoenolpyruvate carboxylase regulation during phosphate starvation, and the integration of glycolysis with nitrogen assimilation. European Journal of Biochemistry 267, 4465-4476
  73. Smith CR, Knowles VL, Plaxton WC (2000) Purification and characterization of cytosolic pyruvate kinase from Brassica napus suspension cell cultures. Implications for the integration of glycolysis with nitrogen assimilation. European Journal of Biochemistry 267, 4477-4485
  74. Grodzinski B, Jiao J, Knowles V, Plaxton W (1999) Photosynthesis & C-partitioning in transgenic tobacco plants deficient in leaf pyruvate kinase. Plant Physiology 120: 887-895
  75. Hodgson RJ, Jia Z, Plaxton WC (1998) Effector induced conformation changes in castor seed cytosolic fructose-1,6-bisphosphatase: A kinetic and fluorometric study. Biochimica Biophysica Acta 1388: 285-294
  76. Hodgson RJ, Plaxton WC (1998) Purification and characterization of cytosolic fructose-1,6-bisphosphate aldolase from endosperm of germinated castor oil seeds. Archives of Biochemistry and Biophysics 355: 189-196
  77. Murley VR, Plaxton WC (1998) Phosphate starvation inducible pyrophosphate-dependent phosphofructokinase occurs in plants whose roots do not form symbiotic associations with mycorrhizal fungi. Physiologia Plantarum 103: 405-414
  78. Rivoal J, Plaxton WC, Turpin DH (1998) Purification and characterization of high and low molecular mass isoforms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii: Kinetic, structural and immunological evidence suggest that the green algal enzyme is distinct from the procaryotic and higher plants enzymes. Biochemical Journal 331: 201-209
  79. Knowles VL, McHugh SG, Hu Z, Dennis DT, Miki BL, Plaxton WC (1998) Altered growth of transgenic tobacco lacking leaf cytosolic pyruvate kinase. Plant Physiology 116: 45-51
  80. Carswell MC, Grant BR, Plaxton WC (1997) Disruption of the phosphate-starvation response of oilseed rape suspension cells by the fungicide phosphonate. Planta 203: 67-74
  81. Law RD, Plaxton WC (1997) Regulatory phosphorylation of banana fruit phosphoenolpyruvate carboxylase by a copurifying phosphoenolpyruvate carboxylase-kinase. European Journal of Biochemistry 247: 642-651
  82. Hu Z-H, Plaxton WC (1996) Purification and characterization of cytosolic pyruvate kinase from leaves of the castor oil plant. Archives of Biochemistry and Biophysics 333: 298-307
  83. Theodorou ME, Plaxton WC (1996) Purification and characterization of pyrophosphate-dependent phosphofructokinase from phosphate starved Brassica nigra suspension cells. Plant Physiology 112: 343-451
  84. Rivoal J, Dunford R, Plaxton WC, Turpin DH (1996) Purification and properties of four PEP carboxylase isoforms from the green alga Selenastrum minutum. Evidence that association of the 102 kDa catalytic subunit with unrelated polypeptides modifies the physical and kinetic properties of the enzyme. Archives of Biochemistry and Biophysics 332: 47-57
  85. Carswell MC, Grant BR, Niere J, Theodorou ME, Plaxton WC (1996) The fungicide phosphonate disrupts the phosphate starvation response of Brassica nigra seedlings. Plant Physiology 110: 105-110
  86. Negm F, Cornel FA, Plaxton WC (1995) Suborganellar localization and molecular characterization of nonproteolytic degraded leukoplast pyruvate kinase from developing castor oil seeds. Plant Physiology 109: 1461-1469
  87. Gottlob-McHugh S, Knowles VL, Blakeley SD, Sangwan RS, Dennis DT, Miki BL, Plaxton WC (1995) Differential expression of cytosolic and plastidic pyruvate kinases in tobacco. Physiologia Plantarum 95: 507-514
  88. Hodgson RJ, Plaxton WC (1995) Effect of polyethylene glycol on the kinetic, fluorometric and oligomeric properties of castor seed cytosolic fructose-1,6-bisphosphatase. FEBS Letters 368: 559-562
  89. Law RD, Plaxton WC (1995) Purification and characterization of a novel phosphoenolpyruvate carboxylase from banana fruit. Biochemical Journal 307: 807-816
  90. Gellatly KS, Moorhead GBG, Duff SMG, Lefebvre DD, Plaxton WC (1994) Purification and characterization of a potato tuber acid phosphatase having significant phosphotyrosyl phosphatase activity. Plant Physiology 106: 223-232
  91. Podestá FE, Plaxton WC (1994) Regulation of carbon metabolism in germinating Ricinus communis cotyledons. I. Developmental profiles for the activity, concentration, and molecular structure of the pyrophosphate- and ATP-dependent phosphofructokinases, PEP carboxylase, and pyruvate kinase. Planta 194: 374-380
  92. Podestá FE, Plaxton WC (1994) Regulation of carbon metabolism in germinating Ricinus communis cotyledons. II. Properties of PEP carboxylase and cytosolic pyruvate kinase associated with the regulation of glycolysis and nitrogen assimilation. Planta 194: 381-387
  93. Cornel FA, Plaxton WC (1994) Characterization of asparaginyl endopeptidase activity in the endosperm of developing & germinating castor oil seeds. Physiologia Plantarum 91: 599-604
  94. Podestá FE, Moorhead GBG, Plaxton WC (1994) Potato tuber PPi-dependent phosphofructokinase: Effects of thiols and polyalcohols on the enzyme's intrinsic fluorescence, activity and quaternary structure in dilute solutions. Archives of Biochemistry and Biophysics 313: 50-57
  95. Moorhead GBG, Hodgson RJ, Plaxton WC (1994) Co-purification of cytosolic fructose-1,6-bisphosphatase and cytosolic aldolase from endosperm of germinating castor oil seeds. Archives of Biochemistry and Biophysics 312: 326-335
  96. Theodorou ME, Plaxton WC (1994) Induction of PPi-dependent phosphofructokinase by phosphate starvation in seedlings of Brassica nigra. Plant, Cell & Environment 17: 287-294
  97. Podestá FE, Plaxton WC (1993) Activation of cytosolic pyruvate kinase by polyethylene glycol. Plant Physiology 103: 285-288
  98. Fischer RS, Bonner CA, Theodorou ME, Plaxton WC, Hrazdina G, Jensen, RA (1993) Response of aromatic pathway enzymes of plant suspension cells to phosphate limitation. Biorganic and Medicinal Chemistry Letters 3: 1415-1420
  99. Theodorou ME, Cornel FA, Duff SMG, Plaxton WC (1992) Phosphate starvation inducible synthesis of the "-subunit of pyrophophosphate: fructose-6-phosphate phosphotransferase in Black Mustard cell-suspension cultures. Journal of Biological Chemistry 267: 21901-21905
  100. Moorhead GBG, Plaxton WC (1992) Evidence that cytosolic aldolase specifically interacts with the ATP- and PPi-dependent phosphofructokinases in carrot storage roots. FEBS Letters 313: 277-280
  101. Podestá FE, Plaxton WC (1992) Plant cytosolic pyruvate kinase: A kinetic study. Biochimica Biophysica Acta 1160: 213-220
  102. Sangwan RS, Singh N, Plaxton WC (1992) Phosphoenolpyruvate carboxylase activity and concentration in the endosperm of developing and germinating castor oil seeds. Plant Physiology 99: 445-449
  103. Sangwan RS, Gauthier DA, Turpin DH, Pomeroy MK, Plaxton WC (1992) Pyruvate kinase isoenzymes from zygotic and microspore-derived embryos of Brassica napus: Developmental profiles and subunit composition. Planta 187: 198-202
  104. Gottlob-McHugh S, Sangwan, RS, Blakeley SD, Vanlerberghe GC, Turpin DH, Ko K, Plaxton WC, Miki B, Dennis DT (1992) Normal growth of tobacco plants in the absence of cytosolic pyruvate kinase. Plant Physiology 100: 820-825
  105. Ko K, Bornemiscza O, Kourtz L, Ko Z, Plaxton WC, Cashmore AR (1992) Isolation and characterization of a cDNA clone encoding a cognate 70 kDa heat shock protein of the chloroplast envelope. Journal of Biological Chemistry 267: 2986-2993
  106. Plaxton WC (1991) Leucoplast pyruvate kinase from developing castor oil seeds: Characterization of the enzyme's degradation by a cysteine endopeptidase. Plant Physiology 97: 1334-1338
  107. Podestá FE, Plaxton WC (1991) Association of phosphoenolpyruvate phosphatase activity with the cytosolic pyruvate kinase of germinating mung beans. Plant Physiology 97: 1329-1333
  108. Podestá FE, Plaxton WC (1991) Kinetic and regulatory properties of cytosolic pyruvate kinase from germinating castor oil seeds. Biochemical Journal 279: 495-501
  109. Duff SMG, Plaxton WC, Lefebvre DD (1991) Phosphate starvation response in plant cells: De Novo synthesis and degradation of acid phosphatases. Proceedings National Academy of Science- U.S.A. 88: 9538-9542
  110. Moorhead GBG, Plaxton WC (1991) High-yield purification of potato tuber pyrophosphate: fructose-6-phosphate 1-phosphotransferase. Protein Expression and Purification 2: 29-33
  111. Blakeley SD, Plaxton WC, Dennis DT (1991) Relationship between the subunits of leucoplast pyruvate kinase from Ricinus communis and a comparison with the enzyme from other sources. Plant Physiology 96: 1283-1288
  112. Duff SMG, Lefebvre DD, Plaxton WC (1991) Purification, characterization, and subcellular localization of an acid phosphatase from black mustard cell-suspension cultures: Comparison with PEP phosphatase. Archives of Biochemistry and Biophysics 286: 226-232
  113. Theodorou ME, Elrifi IR, Turpin DH, Plaxton WC (1991) Effects of P limitation on respiratory metabolism in the green alga Selenastrum minutum. Plant Physiology 95:1089-1095
  114. Moorhead GBG, Plaxton WC (1990) Purification and characterization of cytosolic aldolase from carrot storage roots. Biochemical Journal 269: 133-139
  115. Lefebvre DD, Duff SMG, Fife CA, Julien Inalsingh C, Plaxton WC (1990) Response to phosphate deprivation in Brassica nigra suspension cells: Enhancement of intracellular, cell surface and secreted phosphatase activities compared to increases in Pi absorption rate. Plant Physiology 93: 504-511
  116. Plaxton W.C, Dennis D.T., Knowles V.L. (1990) Purification of leucoplast pyruvate kinase from developing castor bean endosperm. Plant Physiology 94: 1528-1534
  117. Schuller KA, Turpin DH, Plaxton WC (1990) Metabolite regulation of partially purified soybean nodule phosphoenolpyruvate carboxylase. Plant Physiology 94: 1429-1435
  118. Schuller KA, Plaxton WC, Turpin DH (1990) Regulation of phosphoenolpyruvate carboxylase from the green alga Selenastrum minutum: Properties associated with replenishment of TCA cycle intermediates during NH4+ assimilation. Plant Physiology 93: 1303-1311
  119. Vanlerberghe GC, Schuller KA, Smith RG, Feil R, Plaxton WC, Turpin DH (1990) Relationship between NH4+ assimilation rate and in vivo phosphoenolpyruvate carboxylase activity. Regulation of anaplerotic carbon flow in the green alga Selenastrum minutum. Plant Physiology 94: 284-290
  120. Blakeley SD, Plaxton WC, and Dennis DT (1990) The isolation, sequencing & characterization of cDNA clones for the cytosolic isozyme of plant pyruvate kinase. Plant Molecular Biology 15: 665-669
  121. Plaxton WC (1989) Molecular and immunological characterization of plastid and cytosolic pyruvate kinase isozymes from castor oil plant leaf and endosperm. European Journal of Biochemistry 181: 443-451
  122. Plaxton WC, Moorhead GBG (1989) Peptide mapping by CNBr fragmentation using a sodium dodecyl sulfate polyacrylamide minigel system. Analytical Biochemistry 178: 391-393
  123. Knowles VL, Dennis DT, Plaxton WC (1989) Purification and characterization of a novel type of pyruvate kinase from the green alga, Selenastrum minutum. FEBS Letters 259: 130-132
  124.  Duff SMG, Moorhead GBG, Lefebvre DD, Plaxton WC (1989) Phosphate starvation inducible 'bypasses' of adenylate and phosphate dependent glycolytic enzymes in Brassica nigra suspension cells. Plant Physiology 90: 1275-1278
  125. Duff SMG, Lefebvre DD, Plaxton WC (1989) Purification and characterization of a phosphoenolpyruvate phosphatase from Brassica nigra suspension cell cultures. Plant Physiology 90: 734-741
  126. Lin M, Turpin DH, Plaxton WC (1989a) Pyruvate kinase isozymes from a green alga, Selenastrum minutum. I. Purification, physical, and immunological characterization. Archives of Biochemistry and Biophysics 269: 219-227
  127. Lin M, Turpin DH, Plaxton WC (1989b) Pyruvate kinase isozymes from a green alga, Selenastrum minutum. II. Kinetic and regulatory properties. Archives of Biochemistry and Biophysics 269: 228-238
  128. Plaxton WC (1988) Purification of pyruvate kinase from germinating castor bean endosperm. Plant Physiology 86: 1064-1069
  129. Moorhead GBG, Plaxton WC (1988) Binding of glycolytic enzymes to a particulate fraction in carrot and sugar beet storage roots. Dependence on metabolic state. Plant Physiology 86: 348-351
  130. Plaxton WC, Preiss J (1987) Purification and properties of non proteolytic degraded ADPglucose pyrophosphorylase from maize endosperm. Plant Physiology 83: 105-112
  131. Plaxton WC, Storey KB (1986) Glycolytic enzyme binding and metabolic control in anaerobiosis. Journal of Comparative Physiology 156: 635-640
  132. Plaxton WC, Storey KB (1985) Tissue specific isozymes of pyruvate kinase in the channelled whelk, Busycotypus canaliculatum: enzyme modification in response to environmental anoxia. Journal of Comparative Physiology 155: 291-296
  133. Plaxton WC, Storey KB (1985) Purification and properties of aerobic and anoxic forms of pyruvate kinase from the hepatopancreas of the channelled whelk, Busycotypus canaliculatum. Archives of Biochemistry and Biophysics 243: 295-205
  134. Plaxton WC, Storey KB (1984) Purification and properties of aerobic and anoxic forms of pyruvate kinase from red muscle tissue of the channelled whelk, Busycotypus canaliculatum. European Journal of Biochemistry 143: 257-265
  135. Plaxton WC, Storey KB (1984) Phosphorylation in vivo of red muscle tissue pyruvate kinase of the channelled whelk, Busycotypus canaliculatum, in response to anoxic stress. European Journal of Biochemistry 143: 267-272
  136. Plaxton WC, Storey KB (1983) Purification and properties of alanopine dehydrogenase isozymes in the channelled whelk, Busycotypus canaliculatum. Comparative Biochemistry and Physiology 76B: 321-326
  137. Plaxton WC, Storey KB (1982) Alanopine dehydrogenase: purification and characterization of the enzyme from Littorina littorea foot muscle. Journal of Comparative Physiology 149: 57dd-65
  138. Plaxton WC, Storey KB (1982) Tissue specific isozymes of alanopine dehydrogenase in the channelled whelk, Busycotypus canaliculatum. Canadian Journal of Zoology 60: 1568-1572
  139. Storey KB, Miller DC, Plaxton WC, Storey JM (1981) Gas liquid chromatography and enzymatic determination of alanopine and strombine in tissues of marine invertebrates. Analytical Biochemistry 125: 50-58

 

Review Articles & Book Chapters

  1. Plaxton WC (2022) Metabolic Flexibility Helps Plants to Survive Stress. A much updated Web-essay for a web-site that will supplement upcoming 7th Edition of “Plant Physiology” (Taiz, Zeigler, Moller & Murphy, Eds). This Web-essay will augment Chapter 13 ('Respiration') of this widely used undergrad textbook.
  2. Dissanayaka DMSB, Ghahremani M,  Siebers M, Wasaki J, Plaxton WC (2021) Recent Insights into the Metabolic Adaptations of Phosphorus Deprived Plants. Journal of Experimental Botany (Darwin Review) 72: 199–223  (https://doi.org/10.1093/jxb/eraa482)
  3. O'Leary B, Plaxton WC (2020) Multifaceted Functions of  Post-translational Enzyme Modifications in the Control of Plant Glycolysis. Current Opinion in Plant Biology 55: 28–37    
  4. Ghahremani M, Plaxton WC (2020) Phosphoprotein Phosphatase Function of Secreted Purple Acid Phosphatases. In: "Protein Phosphatases and Stress Management in Plants - Functional Genomic Perspective" (GK Pandey, Ed.), Springer (In Press)
  5. Plaxton WC (2019) Avoiding Proteolysis During the Extraction and Purification of Active Plant Enzymes. Plant & Cell Physiology 60: 715–724; article selected as a 'RESEARCH HIGHLIGHT' by PCP editors
  6. Dissanayaka DMSB, Plaxton WC, Lambers H, Siebers M, Marambe B, Wasaki J (2018) Molecular Mechanisms Underpinning Phosphorus-Use Efficiency in Rice  Plant Cell & Environment 41:1483-1496. doi: 10.1111/pce.13191
  7. O'Leary B, Plaxton WC (2017) Mechanisms and Functions of Post-translational Enzyme Modifications in the Organization and Control of Plant Respiratory Metabolism. In. "Plant Respiration: Metabolic Fluxes & Carbon Balance" (G Tcherkez & J Ghashghaie, Eds.). Advances in Photosynthesis and Respiration. Vol. 43, Springer. Chpt. 13, pp. 269-292
  8. Ge S, Champagne P, Plaxton WC, Leite GB, Marazzi F (2017) Microalgal cultivation with waste streams and metabolic constraints to triacylglycerides accumulation for biofuel production. Biofuels, Bioproducts & Biorefining 11:325–343
  9. Plaxton WC (2017) Leucoplast Isolation and Subfractionation. In "Isolation of Plant Organelles and Structures: Methods and Protocols" (NL Talyor & HA Millar, Eds). Methods in Molecular Biology, 1511: 73-81
  10. Ghahremani M, Stigter KA, Plaxton WC (2016) Extraction and characterization of extracellular proteins and their post-translational modifications from Arabidopsis thaliana suspension cell cultures and seedlings: a critical review. Proteomes, 4(3), 25; doi:10.3390/proteomes4030025
  11. O'Leary B, Plaxton WC (2016) Plant Respiration In: eLS (Encyclopedia of Life Sciences). John Wiley & Sons Ltd, Chichester. DOI: 10.1002/9780470015902.a0001301.pub3
  12. Stigter KA, Plaxton WC (2015) Molecular Mechanisms of Phosphorus Metabolism and Transport During Leaf Senescence. Plants 4: 773-798
  13. Lambers H, Finnegan P M, Jost R, Plaxton WC, Shane MW, Stitt M (2015) Phosphorus Nutrition in Proteaceae and Beyond. Nature Plants 1: 1-9
  14. Plaxton WC, Shane MW (2015) The Essential Role of Post-translational Enzyme Modifications in the Metabolic Adaptations of Phosphorus-Deprived Plants. Chpt. 4 of "Phosphorus  Metabolism in Plants" (WC Plaxton & H Lambers, Eds). Annual Plant Reviews. Vol. 48, Wiley; pp. 99-123
  15. Lambers H, Plaxton WC (2015) Phosphorus - Back to the Roots. Chpt. 1 of "Phosphorus  Metabolism in Plants" (WC Plaxton & H Lambers, Eds). Annual Plant Reviews. Vol. 48, Wiley; pp. 3-22
  16. O'Leary B, Plaxton WC (2015) The Central Role of Glutamate and Aspartate in the Post-translational Control of Respiration and Nitrogen-assimilation in Plant Cells. Chpt. 16 of "Amino Acids in Higher Plants" (JPF D'Mello; Ed). CABI International; pp.277-293
  17. Shane MW, Plaxton WC (2015) Preparation of Intracellular and Cell Wall Extracts Optimized for Harsh Hakea. Bio-protocol 5(23): e1678. http://www.bio-protocol.org/e1678
  18. Knowles VL, Plaxton WC (2013) Protein Extraction, Acid Phosphatase Activity Assays, and Determination of Soluble Protein Concentration Bio-protocol 3(17): e889. http://www.bio-protocol.org/e889
  19. Knowles VL, Plaxton WC (2013) Quantification of Total and Soluble Inorganic Phosphate Bio-protocol 3(17): e890. http://www.bio-protocol.org/e890
  20. Plaxton WC, O'Leary B (2012) The Central Role of Phosphoenolpyruvate Metabolism in Developing Oil Seeds. Chpt. 15 of  Seed Development. Omics Technologies Toward Improvement of Seed Quality and Crop Yield. (GK Agrawal & R Rakwal; Eds). Springer.
  21. Veneklass EJ, Lambers H, Bragg J, Finnegan PM, Lovelock CE, Plaxton WC, Price C, Scheible W-R, Shane MW, White PJ, Raven JA (2012) Opportunities for improving phosphorus-use efficiency in crop plants (a Tansley Review). New Phytologist  195: 306-320
  22. Plaxton WC, Tran HT (2011) Metabolic adaptations of phosphate-starved plants. Plant Physiology 156: 1006-1015
  23. O'Leary B, Park J, Plaxton WC (2011) The remarkable diversity of plant phosphoenolpyruvate carboxylase (PEPC): recent insights into the physiological functions and post-translational controls of non-photosynthetic PEPCs. Biochemical Journal 436: 15-44
  24. Tran HT, Hurley BA, Plaxton WC (2010) Feeding hungry plants: The role of purple acid phosphatases in phosphate nutrition. Plant Science 179: 14-27
  25. Plaxton WC (2010) Metabolic Flexibility Helps Plants to Survive Stress. Updated Web-essay for a web-site (http://5e.plantphys.net/) that supplements the 5th Edition of “Plant Physiology” (Taiz & Zeigler). My Web-essay augments Chapter 11 ('Respiration') of this widely used undergraduate textbook.
  26. Bozzo GG & Plaxton WC (2008) The Role of Intracellular and Secreted Purple Acid Phosphatases in Tomato Phosphate Nutrition. In: Tomatoes and tomato products: Nutritional, medicinal and therapeutic properties. (V Preedy & R Watson, eds), Science Publishers, Chpt. 10, pp. 215-234
  27.  Plaxton WC, Podestà FE (2006) The functional organization and control of plant respiration. Critical Reviews in Plant Sciences 25: 159-198
  28. Plaxton WC (2006) Metabolic Flexibility Helps Plants to Survive Stress. Updated Web-essay for a web-site (http://www.plantphys.net/article.php?ch=e&id=124) that supplements the 4th Edition of “Plant Physiology” (Taiz & Zeigler). My Web-essay (14 pgs & 3 figs) augments Chapter 11 ('Respiration') of this widely used undergraduate textbook.
  29. Plaxton WC (2004) Principles of Metabolic Control. In: “Functional Metabolism of Cells: Control, Regulation, and Adaptation” (KB Storey, ed). John Wiley & Sons, Inc., N.Y. pp 1-24
  30. Plaxton WC (2004) Biochemical Adaptations of Phosphate Starved Plants. In: “Encyclopedia of Plant & Crop Science” (R Goodman, ed), Marcel Dekker, N.Y. pp. 976-980
  31. Plaxton WC (2002) Metabolic Flexibility Helps Plants to Survive Stress. Web-essay for a web-site (http://www.plantphys.net/article.php?ch=e&id=124) that supplements the 3rd Edition of “Plant Physiology” (Taiz & Zeigler). My Web-essay (12 pgs & 3 figs) occurs in Chapter 11 ('Respiration') of this undergraduate textbook.
  32. McDonald AE, Grant BR, Plaxton WC (2001) Phosphite (phosphorous acid): Its relevance in the environment and agriculture, and influence on the plant phosphate starvation response. Journal of Plant Nutrition 24:1505 1519
  33. Plaxton WC (1999) Metabolic Aspects of Phosphate Starvation in Plants. In: “Phosphorus in Plant Biology: Regulatory Roles in Molecular, Cellular, Organismic, and Ecosystem Processes”, (J Deikman & J Lynch, eds), ASPP, Rockville MD; pp. 164-176
  34. Plaxton WC, Carswell MC (1999) Metabolic Aspects of the Phosphate Starvation Response in Plants. In: “Plant Responses to Environmental Stresses: From Phytohormones to Genome Reorganization” (HR Lerner, ed.), M. Dekker Inc., NY; pp. 349-372
  35. Plaxton WC (1997) Metabolic Regulation. In: "Plant Metabolism" (DD Lefebvre, DT Dennis, DH Turpin & DB Layzell, eds), Longman Group, pp. 50-68
  36. Plaxton W.C. (1996) The organization and regulation of plant glycolysis. Annual Review of Plant Physiology and Plant Molecular Biology 47: 185-214 (review article)
  37. Theodorou ME, Plaxton WC (1995) Adaptations of Plant Respiratory Metabolism to Nutritional Phosphate Deprivation. In: "Environment and Metabolism: Flexibility and Acclimation" (N Smirnoff, ed), BIOS Scientific publishers, London, U.K., pp. 79-109
  38. Duff SMG, Sarath G, Plaxton WC (1994) The role of acid phosphatases in plant phosphorus metabolism (Invited review). Physiologia Plantarum 90: 791-800 (review article)
  39.  Theodorou ME, Plaxton WC (1993) Metabolic adaptations of plant respiration to nutritional phosphate deprivation. Plant Physiology 101: 339-344
  40. Iglesias AA, Plaxton WC, Podestá FE (1993) The role of inorganic phosphate in the regulation of C4 photosynthesis. Photosynthetic Research 35: 205-211
  41. Plaxton WC (1990) Glycolysis In: "Methods in Plant Biochemistry" (P Lea, ed), Vol. 3, Academic Press, N.Y., pp. 145 173
  42. Plaxton WC (1990) Biochemical Regulation. In: "Advanced Plant Biochemistry and Molecular Biology: Integration and Control of Carbon and Nitrogen Metabolism" (DT Dennis & DH Turpin, eds), Longman Group, pp. 28-44

 

Books Edited

  1. Plaxton WC, McManus MT (co-editors) (2006) Control of Primary Metabolism in Plants. Annual Plant Reviews, Volume 22 (13 chapters), Wiley, U.KControl of Primary Metabolism in Plants book cover
  2. Plaxton WC, Lambers H (co-editors) (2015) Phosphorus Metabolism in Plants. Annual Plant Reviews, Volume 48 (14 chapters), Wiley, U.K.
    Phosphorus Metabolism in Plants book cover

 

Conference Proceedings

  1. Tripodi KE, Plaxton WC (2005) In vivo regulatory phosphorylation of phosphoenolpyruvate carboxylase in developing castor oil seeds. In: “Photosynthesis: Fundamental Aspects to Global Perspectives” (A van der Est & D Bruce, eds); pp. 915-918.
  2. Rivoal J, Trzos S, Gage DA, Plaxton WC, & Turpin DH (1999) Characterization of high and low molecular mass isoforms of phosphoenolpyruvate carboxylase from the green alga Selenastrum minutum. Proceedings of the 1998 International Photosynthesis Congress, pp.151-155
  3. Turpin DH, Rivoal J, Dunford R, Plaxton WC, Huppe HC (1995) Interaction of carbon and nitrogen metabolism in photosynthetic cells: clues from unicellular algae. In: "Photosynthesis: from Light to Biosphere", Volume V (P Mathis, ed), Kluwer Academic Publishers, London, U.K., pp. 353-358
  4. Plaxton WC, Sangwan RS Singh N, Gauthier DA, Turpin DH (1993) Phosphoenolpyruvate metabolism of developing oil seeds. In: "Seed oils for the future" (SL MacKenzie and DC Taylor, eds), American Oil Chemists Society Press, Champaign, IL, pp. 35-43
  5. Turpin DH, Weger HG, Smith RG, Plaxton WC, Lin M (1990) Interactions between respiration and N assimilation during photosynthesis. In: "Inorganic nitrogen metabolism" (W Ulrich, ed.), Springer Verlag, Switzerland, pp. 124-130
  6. Preiss J, Bloom M, Morell MK, Knowles VL, Plaxton WC, Okita T, Larsen R, Harmon A, Putnam Evans C. (1987) Regulation of starch synthesis, enzymological and genetic studies. In: "Tayloring genes for crop improvement: An agricultural perspective" (T Kosuge, J Harada, G Bruening and A Hollander, eds), Plenum Publishing Corp., N.Y., pp. 133 152