Please enable javascript to view this page in its intended format.

Queen's University

Yuxiang Wang - Associate Professor

Wang.jpg Research: My research focuses on the regulation of metabolism in animals. Lower vertebrates, especially fish, are the model used to study how animals cope with metabolic disequilibrium that results from different physiological states (exercise and stress) or changing environments (oxygen, temperature, ammonia, and anthropogenic pollutants).

The maintenance of a delicate energy balance is a constant challenge any organism must face in order to survive. This intricate metabolic regulation allows organisms to maintain an equilibrium between energy demand and supply. In higher organisms, such as those in the animal kingdom, the fine tuning of the various functions over the course of evolution has been partially achieved through the compartmentalization of the system. In essence, it is vital to maintain an efficient energy distribution and re-distribution system among different tissues, cells, and sub-cellular organelles. Glycolysis is an ancient metabolic pathway present in essentially all organisms. Lactate, the end-product of anaerobic glycolysis, is formed when tissues must function under oxygen limiting conditions. Some tissues, such as muscle, red blood cells and tumor cells, rely heavily on this pathway to generate ATP even under normal physiological conditions. Lactate can also be taken up and utilized by a wide variety of tissues and is thus considered to be an important metabolic intermediate substrate rather than just an anaerobic end-product. In fish, lactate metabolism and transport are handled differently in comparison to mammals. My research will shed light on the molecular and physiological regulation of the transmembrane lactate transport system and its links to other metabolites.

»» Lab Website »« email: »« telephone: 613-533-6134 ««

Some Recent Publications: 
61. Razavi Roxanna N., Mingzhi Qu, Dongmei Chen, Yang Zhong, Wenwei Ren, Y.X. Wang, and Linda M. Campbell§ (2013) Evidence of algal biodilution in an ecoregion under high mercury deposition Environ. Sci Tech (in review)

60. Razavi Roxanna N., Mingzhi Qu, Binsong Jin, Wenwei Ren, Y.X. Wang, and Linda M. Campbell (2013) Mercury biomagnification in a stocked subtropical reservoir of eastern China, Ecotox. (in review)

59. Birceanu, Oana, Lisa A. Sorensen, Matthew Henry, Grant B. McClelland, Y.X. Wang, and Michael P. Wilkie (2013) The effects of the lampricide 3-trifluoromethyl-4-nitrophenol (TFM) on fuel stores and ion balance in a non-target fish, the rainbow trout (Oncorhynchus mykiss). Comp Biochem. Physiol. C. (in press)

58. Dhillon R.S., L Yao, V Matey, BJ Chen, SJ Fu, ZD Cao, CJ Brauner, YX Wang and JG Richards (2013) Interspecific differences in hypoxia-induced gill remodeling in carp. Physiol. Biochem. Zool. (in press)

57. Davies, R*, K Mathers, K Bremer, Y Wang, CD Moyes. (2013) Influence of hybridization on mitochondrial function in sunfish. Physiol. Biochem. Zool. 85:321-331

56. Andrade J.A.*, G.G. Goss, A. Dowker, A. Bonen, P.M. Schulte  and  Y.X. Wang (2012) Characterization of a novel monocarboxylate transporter in killifish (Fundulus heteroclitus) KFMCT. Comp. Biochem. Physiol. A (to be submitted)

55. Speers-Roesch B., J.G. Richards, C.J. Brauner, A.P. Farrell, A.J.R. Hickey, Y.X. Wang and G.M.C. Renshaw (2012) Hypoxia tolerance in elasmobranchs. II. Cardiovascular function and tissue metabolic responses during progressive and relative hypoxia exposures。J. Exp. Biol.  215:103-114 
54. Speers-Roesch B., J.G. Richards, C.J. Brauner, A.P. Farrell, A.J.R. Hickey, Y.X. Wang and G.M.C. Renshaw (2012) Hypoxia tolerance in elasmobranchs. I. Critical oxygen tension as a measure of blood oxygen transport during hypoxia exposure。 J. Exp. Biol. 215:93-102

53. Hickey A.J.R, G.M.C. Renshaw, B. Speers-Roesch, J.G. Richards, Y.X. Wang, A.P. Farrell and C. J. Brauner (2012) A radical approach to beating hypoxia: depressed free radical release from heart fibres of the hypoxia-tolerant epaulette shark (Hemiscyllum ocellatum) J. Comp Phyiol. (B) 182:91-100

52. Brauner, C.J., V. Matey, W. Zhang, J.G.  Richards, R. Dhillon, Z-D. Cao, Y. X Wang, and S-J Fu* (2011) Gill remodeling during sustained exercise and the effect on subsequent swimming performance in the European crucian carp. Physiol Biochem. Zool. 84:535-542

51. Fu S-J*, C. J. Brauner, Z-D Cao, J.G. Richards, J-L Peng, R Dhillon and Y.X Wang (2011) The effect of acclimation to hypoxia and sustained exercise on subsequent hypoxia tolerance and swimming performance in goldfish (Carassius auratus). J. Exp Biol. 214:2080-2088

50. Davies R.*, C. D. Moyes, Y.X. Wang (2011) Intra- and Inter-specific variation in metabolic gene expression in relationship to environmental hypoxia. Comp. Biochem. Physiol.A, 159:25-31

49. Birceanu O., G.B. McClelland,Y.X. Wang, J.C. L. Brown, M. P. Wilkie (2011)  The lampricide 3-trifluoromethyl-4-nitrophenol (TFM) uncouples mitochondrial oxidative phosphorylation in both sea lamprey (Petromyzon marinus) and TFM-tolerant rainbow trout (Oncorhynchus mykiss). Comp. Biochem. Physiol. C, 153:342-349

48. O’Bryan D.M., Z. Xie*, Y.X. Wang, J. Du, C.J. Brauner, J.G. Richards, C.M. Wood, X.-Q. Chen and B. W. Murray (2010) Phylogeography and conservation genetics of Lake Qinghai scaleless carp (Gymnocypris przewalskii) J. Fish Biol. 77(9):2072-2092

47. Chen B-J Z-D CAO, S-J Fu* and Y.X. WANG (2010) Temperature effect on rest metabolic rate and hypoxia tolerance in Chinese bream Parabramis pekinensis Chinese J. Zool. 45(5):1-6

46. Gonzalez R. J., C.J. Brauner, Y.X. Wang, J.G. Richards, M.L. Patrick, W. Xu, and A.L. Val.  (2010) Impact of ontogenetic changes in branchial morphology Arapaima gigas. Physiol Biochem. Zool. 83(2):322-332

45. Liu, Y., Z-D Cao, S-J Fu*, J-L Peng and Y.X. Wang (2009) The effect of exhaustive chasing training and detraining on swimming performance in juvenile darkbarbel catfish (Peltebagrus vachelli) J. Comp. Physiol. 179(7): 847-855

44. Cao, Y. B.*, X. Q. Chen, S. Wang*, X. C. Chen, and Y. X. Wang, J. P. Chang, and J. Z. Du. (2009) Growth hormone and insulin-like growth factor of naked carp (Gymnocypris przewalskii) in Lake Qinghai: Expression in different water environments. Gen.Comp.Endocr. 161: 400-406,

43. NGAN, A* and Y.X. WANG  (2009) Tissue-specific transcriptional regulation of monocarboxylate transporters (MCTs) during short-term hypoxia in zebrafish (Danio rerio). Comp Biochem Physiol. B, 154(4):396-405

42. Birceanu O., G.B. McClelland; Y.X. Wang; and M. P. Wilkie (2009) A failure of ATP supply to match ATP demand: the mechanism of toxicity of the lampricide, 3-trifluoromethyl-4-nitrophenol (TFM), used to control sea lamprey (Petromyzon marinus) populations in the Great Lakes. Aqua. Tox. 94:265-274

41. Zeng L-Q, Z-D Cao, S-J Fu*, J-L Peng and Y.X. Wang (2009) Effect of temperature on swimming performance in juvenile southern catfish (Silurus meridionalis Chen).  Comp. Biochem. Physiol. A 153(2):125-130

40. Dhillon R.S.*, Y.X. Wang, and B. L. Tufts (2009) Charactrization and expression of a myosin heavy chain in juvenile walleye (Sander vitreus).  J. Fish Biol. 75(5):1048-1062

39. Fu S-J*, L-Q Zeng, X-P Pang, X-M Li, Z-D Cao, J-L Peng  and Y.X. Wang (2009) The behavioral, digestive and metabolic strategies in four fishes with different foraging behavior J. Exp. Biol. 212:2296-2302

38. Fu, S-J.*, L-Q Zeng, X-M Li, X. Peng, Z-D Cao, J-L Peng, and Y.X. Wang (2009) Effect of meal size on excess post-exercise oxygen consumption in fishes with different locomotive and digestive performance. J. Comp. Physiol (B) 179(4):509-517

37. Madison, B.N.*, R.S. Dhillon*, B.L. Tufts and Y.X. Wang (2009) Exposure to low concentrations of dissolved ammonia promotes growth rate in walleye Sander vitreus. J. Fish Biol. 74:872-890

36. Wang Y-S, Z-D Cao, S-J Fu* and Y.X Wang (2008) Thermal tolerance of juvenile Silurus meridionalis (Chen). Chinese J. Ecol. 27(12):1-5

35. Cao YB*, XQ Chen, S. Wang*, and Y.X. Wang, and J.-Z Du (2008) Evolution and regulation of the downstream gene of hypoxia-inducible factor 1 alpha (HIF-1α) in naked carp (Gymnocypris przewalskii) in Lake Qinghai of China. Mol. Evol. 67(5):570-580

34. Fu, S-J*, Z-D. Cao,J-L. Peng , and Y. X. Wang (2008) Is peak postprandial oxygen consumption positively related to growth rate and resting oxygen consumption in a sedentary catfish? J. Fish Biol. 73:692-701

33. Matey, V., J. G. Richards, Y.X. Wang, C. M. Wood, J. Rogers, R. Davies*, B. W. Murray, X-Q Chen, J-Z Du and C. J. Brauner (2008) The effect of hypoxia on gill morphology and ionoregulatory status in the Lake Qinghai scaleless carp, Gymnocypris przewalskii J. exp. Biol. 211:1063-1074

32. Wood C.M., J-Z. Du, J. Rogers, C. J. Brauner, J.G. Richards, J.W. Semple*, B. W. Murray, X-Q Chen, and Y.X. Wang (2007) Przewalski’s Naked Carp (Gymnocypris przewalskii): An Endangered Species Taking a Metabolic Holiday in Lake Qinghai, China, Physiol Biochem. Zool. 80(1):59-77

31. Wilkie M.P., J. F. Claude, A. Cockshutt, J. A. Holmes, Y.X. Wang, J. H. Youson and P. J. Walsh (2006) Shifting Pattern of Nitrogen Excretion and Amino Acid Catabolism Capacity During the Life Cycle of the Sea Lamprey (Petromyzon marinus). Phyiol. Biochem. Zool. 79(5):885-898

30. Richards J.G., C.J. Brauner, R.J. Gonzalez, M.L. Patrick, P.M. Schulte., A.R. Choppari-Gomes., A.L. Val,  V.M. Almeida-Val., and Y.X. Wang. (2007) Metabolic and ionoregulatory responses of the Amazonian cichlid, Astronatus ocellatus, to severe hypoxia.  Comp. Physiol. 177(3):361-374

29. Madison, B.N.*and Y.X. Wang (2006) Haematological consequences of acute sub-lethal nitrite exposure in walleye (stizostedion vitreum) Aqua Toxicol. 79:16-23

28. Xie Z-Y*, J-Z. Du, X-Q Chen and Y.X. Wang, and B.W. Murray (2005) The Significance of Mitochondria Control Region (D-Loop) in Intraspecific genetic differentiation of fish, Hereditas (Beijing) 28(3) 262-268.

27. Chen, X-Q., J-Z. Du, and Y. X. Wang (2004) Regulation of hypoxia-induced release of corticotropin-releasing factor in the rat hypothalamus by norepinephrine. Regulatory Peptides 119: 221-228.

26. Brauner, C.J., T. Wang, R. Gonzalez, Y.X. Wang, R.G. Richards, N.J. Bernier, W. Xu, M. Patrick and A.L. Val. (2004) Limited extracellular but complete intracellular acid-base regulation during environmental hypercapnia in the armoured catfish, Liposarcus pardalis. J. Exp. Biol. 207: 3381-3391.

25. Wilkie M.P., S. Turnbull, J. Bird, Y.X. Wang, J.F. Claude and J.H. Youson (2004) Lamprey parasitism of sharks and teleosts: high capacity urea excretion in an extant vertebrate relic. Comp. Biochem. Physiol A. 138(4): 485-492

24. Wang, Y.X., R.J. Gonzalez, M.L. Patrick, M. Grosell, C.G. Zhang, J.Z. Du,  P.J. Walsh, C.M. Wood Unusual physiology of Lake Qinghai scaleless carp, Gymnocypris przewalskii (2003) Comp. Biochem. Physiol. A. 134: 409-421.

23. Furimsky, M.*, S.J. Cooke, C.D. Suski, Y.X. Wang and B.L. Tufts (2003). Respiratory and circulatory effects of hypoxia in largemouth bass (Micropterus salmoides) and small mouth bass (Micropterus dolomieu): an application to “live-release” fishing tournament. Trans. Am. Fish. Soc. 132: 1065-1075.

22. Wang, Y.X., D. Miskovic, M. Tonouchi*, and A. Bonen (2003) Thyroid hormone (T3) regulates monocarboxylate transporter isoforms (MCT1 and MCT4) and mRNA expression in rat skeletal and cardiac muscle. Am. J. Physiol. 285: E622-E628.

21. Veauvy, C.*, Y.X. Wang, Perez-Pinzon G.P., P.J. Walsh (2001) Comparison of ammonia effect on brain metabolism in rat and toadfish. Am. J. Physiol. 283: R598-R603.

20. Walsh, P.J., Y.X. Wang, C.E. Campbell, G. DeBoeck and Chris M. Wood (2001). Patterns of nitrogenous waste excretion and gill urea transporter mRNA expression in various species of marine fish. Mar. Biol. 139:839-844.

19. Hatta, H., M. Tounuchi*, D. Miskovic, Y.X. Wang, J. Heikkila and A. Bonen (2001). Tissue specific and isoform specific change in MCT1 and MCT4 in heart and soleus muscle during a 1 year period. Am. J. Physiol. Endocrinol. Metab. 281:E749-E756.

18. Wood, C.M., D.M. McDonald*, J. Warne, R. Balment, Y.X. Wang, and P.J. Walsh (2001). Do circulating plasma AVT and/or control pulsatile urea excretion in the gulf toadfish (Opsanus beta)? Comp. Biochem. Physiol. A. 129:859-872

17. Laurent, P., C.M. Wood, Y.X. Wang, S.F. Perry, K.M. Gilmour, P. Pärt and P.J. Walsh (2001) Intracellular vesicular trafficking in the gill epithelium of urea excreting fish. Cell & Tissue Res. 303:197-210.

16. McDonald, M.D.*, C.M. Wood, Y.X. Wang and P.J. Walsh (2000) Differential branchial and renal handling of urea, acetamide and thiourea in the gulf toadfish, Opsanus beta: evidence for two transporters. J. exp. Biol. 203:1027-1037.

15. Wang, Y.X. and P.J. Walsh (2000) High ammonia tolerance in fishes of the family Batrachoididae (toadfish and midshipmen). Aqua. Toxicol. 50:205-219.

14. Walsh, P.J., M.Hertz, M. Medina, G.G. Goss, V. Vincek, Y.X. Wang, C. E. Campell, G.J. Cooper, C.M. Wood and C.P. Smith (2000) Molecular characterisation of a urea transporter in the gills of the gulf toadfish, Opsanus beta. J. exp. Biol. 203:2357-2364.

13. Kong,H., N.Kahatapitiya, K. Kingsley, W.L. Salo, P.M. Anderson, Y.X. Wang and P. J. Walsh (2000) Induction of carbamoyl phosphate synthetase III and glutamine synthetase mRNA during confinement stress in gulf toadfish (Opsanus beta). J. exp. Biol. 203:311-317.

12. Wilkie, M.P., Y.X. Wang, P.J. Walsh and J.H. Youson (1999) Nitrogen waste excretion by the larvae of a phylogenetically ancient vertebrates: the sea lamprey (Petromyzon marinus). Can. J. Zool. 77:707-715.

11. Walsh, P.J. and Y.X. Wang (1998) Re-examination of nitrogenous waste excretion in several marine teleost fishes. Bull. Mt. Desert Island Biological Lab.

10. Wang, Y.X., R.P. Henry, P.W. Wright*, G.J.F. Heigenhauser and C.M. Wood (1998) Respiratory and metabolic roles of carbonic anhydrase in post-exercise trout skeletal muscle. Am. J. Physiol. 275:R1766-R1779.

9. Wang, Y.X. and Bergman H.L. (1997) Report on South Platte River Segment 15 Studies: Laboratory studies to determine the response of fish species to diel fluctuations in dissolved oxygen (prepared for Metro Denver Wastewater Reclamation District).  

8. C.M. Wood and Y.X. Wang (1999) Lactate, H+ and ammonia transport and distribution in rainbow trout white muscle after exhaustive exercise. In: S.E.B Seminar Series: Regulation of Tissue pH in Plants and Animals: a reappraisal of current techniques pp.99-124 (Ed. E.W. Taylor, S. Egginton, and J.A. Raven), London, Cambridge Univ. Press.

7. Henry, R.P., Y.X. Wang and C.M. Wood (1997) Carbonic anhydrase facilitates CO2 and NH3 transport across the sarcolemma of trout white muscle. Am. J. Physiol. 272: R1754-R1761.

6. Wang, Y.X., P.W. Wright*, G.J.F. Heigenhauser and C.M. Wood (1997) Lactate transport by perfused rainbow trout white muscle: kinetic characteristics and sensitivity to inhibitors. Am. J. Physiol. 272: R1577-R1587.

5. Wang, Y.X., G.J.F. Heigenhauser and C.M. Wood (1996) Ammonia movement and distribution after exercise across white muscle cell membranes in rainbow trout. Am. J. Physiol. 271: R738-R750.

4. Wang, Y.X., G.J.F. Heigenhauser and C.M. Wood (1996) Lactate, metabolic H+ transport and distribution after exercise across white muscle cell membranes in rainbow trout. Am. J. Physiol. 271: R1239-R1250.

3. Wang, Y.X., G. Heigenhauser and C.M. Wood (1994) Integrated responses to exhaustive exercise and recovery in vivo in rainbow trout white muscle: acid-base, phosphagen, carbohydrate, lipid, and electrolyte metabolism. J. Exp. Biol. 195:227-258

2. Wang, Y.X., M.P. Wilkie, G. Heigenhauser and C.M. Wood (1994) The analysis of metabolites in rainbow trout white muscle: a comparison of different sampling and processing methods. J. Fish Biol. 45:855-873.

1. Wang, Y.X. and Evans, R.D. (1993) Influence of calcium concentrations on cadmium uptake by freshwater mussels Elliptio complanata. Can. J. Fish. Aquat. Sci.  50 (12): 2591-2596.

Kingston, Ontario, Canada. K7L 3N6. 613.533.2000